[ExI] Whole-organ cryopreservation (AND RESTORATION!) successful.

Rafal Smigrodzki rafal.smigrodzki at gmail.com
Wed Apr 30 01:30:26 UTC 2008

On Tue, Apr 29, 2008 at 8:48 PM, Bret Kulakovich
<bret at bonfireproductions.com> wrote:
> Perhaps my subject wasn't well written?
> Or did everyone know about the hearts in question all being perfused,
> frozen, thawed, and reperfused?

### Yes, the hearts were reperfused but not reimplanted. The true test
of the technology would have to include reimplantation and observation
of function for at least 2 - 3 weeks after reimplantation, since
apoptosis is an active process taking up to a few days, and it may be
followed by scar formation which can take weeks. Apoptotic death is an
important limitation on the functioning of frozen and thawed tissues.

I can send the pdf to interested parties off-list.


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