[ExI] crispr question

[Rafal Smigrodzki]

On Thu, Apr 7, 2016 at 1:01 AM, spike <spike66 at att.net> wrote:

>
>
> As you understand it and extrapolate to a reasonable estimate of your
> lifespan, have we any reason to think that if we had an organism’s complete
> genome, we could synthesize that file into a DNA strand using CRISPR or any
> other reasonably foreseeable technology?
>
>
>
### As Anders noted, CRISPR isn't really a good method for de novo DNA
synthesis, however, it might come in useful in polishing steps of large
genome synthesis. Fascinating advances are being made in the in vitro
manufacture of long and very long sequences:

http://www.businesswire.com/news/home/20160209005653/en/Gen9-Develops-Multiplex-Synthesis-Platform-DNA-Assembly

but it is still likely that human genome-size constructs will need in vivo
assembly and proofreading, and that's where a CRISPR step is likely to play
a role.

Rafał
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lists.extropy.org/pipermail/extropy-chat/attachments/20160407/6c8a48d8/attachment.html>