<div dir="auto"><br><br><div class="gmail_quote" dir="auto"><div dir="ltr" class="gmail_attr">On Sat, Sep 5, 2020, 12:33 AM spike jones via extropy-chat <<a href="mailto:extropy-chat@lists.extropy.org">extropy-chat@lists.extropy.org</a>> wrote:</div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div lang="EN-US" link="blue" vlink="purple"><div class="m_9053401575491779666WordSection1"><div><p class="MsoNormal" style="margin-bottom:12.0pt">Keith was the one who hipsterized me to how viruses don’t have DNA, they have RNA. But I had heard that polymerase chain reaction is not a reliable amplifier of RNA, only DNA. </p></div></div></div></blockquote></div><div class="gmail_quote" dir="auto"><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div lang="EN-US" link="blue" vlink="purple"><div class="m_9053401575491779666WordSection1"><div><p class="MsoNormal" style="margin-bottom:12.0pt"></p></div></div></div></blockquote></div><div dir="auto">Two comments, many viruses do have DNA. CV-19 is not one of them though. </div><div dir="auto"><br></div><div dir="auto">There are no issues with RT-PCR used for RNA detection in terms of protocol. There's just an extra step in the beginning using reverse transcriptase to get a DNA strand to amplify from the RNA, and then it's just like regular PCR. </div><div class="gmail_quote" dir="auto"><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div lang="EN-US" link="blue" vlink="purple"><div class="m_9053401575491779666WordSection1"><p class="MsoNormal" style="margin-bottom:12.0pt">a? If so, it would explain why the people who use the covid tests every day say they are notoriously unreliable: they are using PCR to amplify RNA. My nurse cousin thinks they have an unacceptably high false positive rate. <u></u><u></u></p><p class="MsoNormal" style="margin-bottom:12.0pt">spike</p></div></div></blockquote></div><div dir="auto"><br></div><div dir="auto">It's actually very accurate, the potential issue is with sensitivity being too high. Even a very small amount of RNA can be detected that may not be biologically active. </div><div dir="auto"><br></div><div dir="auto">Sensitivity issue:</div><div dir="auto"><a href="https://www.advisory.com/daily-briefing/2020/09/01/covid-tests">https://www.advisory.com/daily-briefing/2020/09/01/covid-tests</a><br></div><div dir="auto"><br></div><div dir="auto">Protocol:</div><div dir="auto"><a href="https://www.iaea.org/newscenter/news/how-is-the-covid-19-virus-detected-using-real-time-rt-pcr">https://www.iaea.org/newscenter/news/how-is-the-covid-19-virus-detected-using-real-time-rt-pcr</a><br></div><div class="gmail_quote" dir="auto"><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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