[ExI] Another step towards uploading

Rafal Smigrodzki rafal.smigrodzki at gmail.com
Wed Oct 9 05:04:49 UTC 2013


On Tue, Oct 8, 2013 at 1:41 PM, John Clark <johnkclark at gmail.com> wrote:

> Thanks Max, I'll have more comments about that article in a few days but
> first a quick question, Mr. Wolf keeps saying things like:
>
>>  > In neural cryobiology, on the other hand, it is possible to subject the
>> cryopreserved brain tissue to both a viability test and (subsequently) to
>> ultrastructural examination.
>
>
> What is he talking about?  Today with the absence of advanced Nanotechnology
> how can you give a viability test to a block of tissue frozen harder than a
> brick?
>
### You can thaw a piece and try to record electric activity,
dissociate the tissue and try to culture surviving cells, look for
evidence of metabolism (oxygen, glucose consumption, carbon dioxide
production). This list is roughly in the diminishing order of
viability.

Rafal



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